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Abstract

α-Amylase is one of important enzymes being used in various fields. In response to the needs of thermostable a-amylase in practical applications, we established a clone of recombinant B. subtilis being able to produce a thermostable a-amylase. The amyQ gene encoding a thermostable a-amylase from Bacillus amyloliquefaciens was cloned to an expression vector, transformed into and overexpressed in B. subtilis. The expression of amyQ gene in the recombinant B. subtilis clone was qualitatively and quantitatively tested basing on a-amylase activity. The overexpression of a-amylase gene was confirmed by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE).



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Issue: Vol 7 No 2 (2004)
Page No.: 31-36
Published: Feb 29, 2004
Section: Article
DOI: https://doi.org/10.32508/stdj.v7i2.3159

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Creative Commons License

Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 How to Cite
Vi Ca, H., Phuong Thao, D., & Linh Thuoc, T. (2004). TRANSFORMATION AND OVEREXPRESSION OF GENE ENCODING THERMOSTABLE A-AMYLASE IN Bacillus subtilis. Science and Technology Development Journal, 7(2), 31-36. https://doi.org/https://doi.org/10.32508/stdj.v7i2.3159

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