TRANSFORMATION AND OVEREXPRESSION OF GENE ENCODING THERMOSTABLE A-AMYLASE IN Bacillus subtilis

Abstract

α-Amylase is one of important enzymes being used in various fields. In response to the needs of thermostable a-amylase in practical applications, we established a clone of recombinant B. subtilis being able to produce a thermostable a-amylase. The amyQ gene encoding a thermostable a-amylase from Bacillus amyloliquefaciens was cloned to an expression vector, transformed into and overexpressed in B. subtilis. The expression of amyQ gene in the recombinant B. subtilis clone was qualitatively and quantitatively tested basing on a-amylase activity. The overexpression of a-amylase gene was confirmed by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE).