ESTABLISHING A YEAST Saccharomyces cerevisiae CLONE EXPRESSING GFP (GREEN FLUORESCENT PROTEIN) ON THE CELL SURFACE

Abstract

GFP is a fluorescent protein from jellyfish Aequorea victoria. The luminescence of GFP is occurred based on an energy transfer from another protein without requirement of the role of any cofactor or enzyme. GFP has been used in many areas such as medicine, biotechnology, cell biology, ... In this research, by using DNA recombinant technology, we succeeded in expressing gfp gene on cell surface of the yeast Saccharomyces cerevisiae. Gfp gene is cloned onto the expression vector pICASI downstream of the GAPDH promoter, the secretion signal sequence and upstream of 3’ half of α-agglutinin gene. The presence of GFP on the yeast recombinant clone Saccharomyces cerevisiae was proved by fluorescent microscopic observation. By fusing in frame of a target gene with the gfp gene, the GFP-displaying system on yeast cell surface can be used as a reporter system for the expression of any foreign genes on the yeast cell surface.