Abstract

The glucoamylase gene-expressing plasmid PGA11 contains a fused DNA of glucoamylase coding gene from the mold Rhizopus oryzae linked at the 5' end with the yeast secreting signal sequence sss and at the 3' end with the coding sequence of the C-terminal domain of a-agglutinin under the control of the inducible GAPDH promoter. The transformation of PGA11 into the yeast Saccharomyces cerevisiae MT8-1 resulted in the recombinant clone MT-PGA which possesses the hydrolyzing activity of starch to form a clear halo circle surrounding the yeast colony and the ability to directly ferment stacrh into alcohol. Practical application of the yeast recombinant clone in starch transformation was discussed.