Open Access 
Downloads
Download data is not yet available.
Abstract
The glucoamylase gene-expressing plasmid PGA11 contains a fused DNA of glucoamylase coding gene from the mold Rhizopus oryzae linked at the 5' end with the yeast secreting signal sequence sss and at the 3' end with the coding sequence of the C-terminal domain of a-agglutinin under the control of the inducible GAPDH promoter. The transformation of PGA11 into the yeast Saccharomyces cerevisiae MT8-1 resulted in the recombinant clone MT-PGA which possesses the hydrolyzing activity of starch to form a clear halo circle surrounding the yeast colony and the ability to directly ferment stacrh into alcohol. Practical application of the yeast recombinant clone in starch transformation was discussed.
Author's Affiliation
Article Details
Issue: Vol 5 No 7&8 (2002)
Page No.: 36-43
Published: Aug 31, 2002
Section: Article
DOI: https://doi.org/10.32508/stdj.v5i7&8.3426
Copyright Info
Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
How to Cite
Phuong Thao, D., & Linh Thuoc, T. (2002). ESTABLISMENT OF A RECOMBINANT YEAST SACCHAROMYCES CEREVISIAE EXPRESSING GLUCOAMYLASE-CODING GENE. Science and Technology Development Journal, 5(7&8), 36-43. https://doi.org/https://doi.org/10.32508/stdj.v5i7&8.3426
Download Citation
Cited by
Article Statistics
HTML = 1019 times
Download PDF = 1018 times
Total = 1018 times
Download PDF = 1018 times
Total = 1018 times
Most read articles by the same author(s)
- Vo Minh Tri, Tran Linh Thuoc, EXAMINATION ON CONDITIONS OF ATP - DEPENDENT BIOLUMINESCENCE REACTION FOR RAPID QUANTIFICATION OF MICROBES , Science and Technology Development Journal: Vol 5 No 7&8 (2002)
- Nguyen Duc Hoang, Phan Thi Phuong Trang, Tran Linh Thuoc, Mitsuyoshi Ueda, Atsuo Tanaka, CONSTRUCTION OF A PLASMID EXPRESSING EYFP IN THE YEAST CYTOPLASMIC FRACTION UNDER GLUCOSE INDUCTION , Science and Technology Development Journal: Vol 5 No 7&8 (2002)
