Abstract

The GAPDH (glyceraldehyde 3-phosphate dehydrogenase) promoter and the EYFP gene (Enhanced Cyan Fluorescence Protein) were cloned and introduced into the plasmid PRS406 containing the 2um DNA of yeast genome to produce an expressing vector named as pR2GY which can be induced by glucose in the yeast cell. The constructed vector could express EYFP in the yeast Saccharomyces cerevisiae in the medium containing glucose. The fluorescence of EYFP in the yeast was confirmed under fluorescence microscopic observation and by measuring the fluorescent intensity of the cytosol fraction of the yeast cells.