Open Access 
Downloads
Download data is not yet available.
Abstract
In this study, we report results on the expression and purification of recombinant T4 DNA ligase. Plasmid pET16b-T4Dnl contains the gp30 gene which encodes for T4 DNA ligase. The target protein is fused with 10xHis tag to facilitate the purification and recovery. pET16b-T4Dnl was transformed into E. coli BL21(DE3) and then induced the expression of 10xHis-T4Dnl by IPTG. The recombinant protein was purified by Ni-NTA chromatography and confirmed by SDS-PAGE and Western blot. The activity of purified protein was tested by joining DNA λ/HindIII.
Author's Affiliation
Article Details
Issue: Vol 14 No 3 (2011)
Page No.: 73-79
Published: Sep 30, 2011
Section: Natural Sciences - Research article
DOI: https://doi.org/10.32508/stdj.v14i3.1991
Copyright Info
Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
How to Cite
Duong, D., Luong, D., Nguyen, H., Tran, H., Dang, T., & Tran, T. (2011). EXPRESSION AND PURIFICATION OF RECOMBINANT T4 DNA LIGASE IN E. COLI. Science and Technology Development Journal, 14(3), 73-79. https://doi.org/https://doi.org/10.32508/stdj.v14i3.1991
Download Citation
Cited by
Article Statistics
HTML = 2395 times
Download PDF = 1819 times
Total = 1819 times
Download PDF = 1819 times
Total = 1819 times
Most read articles by the same author(s)
- Hanh Vu Bich Dang, Thuoc Linh Tran, Huyen Vu Xuan Dang, THE STUDY ON HEAVY METAL REMOVED BY BACILLUS SUBTILIS WITH POLYHISTIDINE 6X ON CELL SURFACE , Science and Technology Development Journal: Vol 13 No 3 (2010)
- Vinh Ngoc Tran, Quy Cam Vo, Thuoc Linh Tran, PREDICTION OF B-CELL DISCONTINUOUS EPITOPES ON MATRIX PROTEIN OF H5N1 VIRUS , Science and Technology Development Journal: Vol 12 No 9 (2009)
- Van Hai Van, Thuy Thi Thanh Le, Phuong Thi Ngoc Cao, Bich Thi Vu, Thuoc Linh Tran, INDENTIFYING FUNCTIONALLY CONSERVED REGIONS AND PREDICTING T-CELL EPITOPES ON PROTEINS OF INFLUENZA A VIRUS , Science and Technology Development Journal: Vol 12 No 9 (2009)
- Hieu Thi Phuong Nguyen, Linh Thuy Lien, Thuoc Linh Tran, CLONING AND EXPRESSING HG-CSF (HUMAN GRANULOCYTE COLONY STIMULATING FACTOR) BEING FUSED WITH FERRITIN HEAVY-CHAIN IN E. COLI , Science and Technology Development Journal: Vol 12 No 9 (2009)
