Open Access 
Downloads
Abstract
Although discontinuous epitopes make up 90% of total number of B-cell epitopes, however, because of difficulties in the development of method for their prediction, most of the B-cell epitope prediction methods today focus on continuous epitopes. To serve for the development of vaccine against H5N1 virus, we have been studying on in silico prediction of T- and B-cell continuous as well as B-cell discontinuous epitopes on H5N1 viral antigens. In this study, using the homology modeling method, we have generated structures of matrix protein of the H5N1 virus and predicted B-cell discontinuous epitopes. 60 out of 72 predicted residues were similar with those reported by the CEP method (Conformational Epitope Prediction). All predicted aminoacid residues were hydrophilic, polar, electrically charged and located on the surface of the antigen structures.
Issue: Vol 12 No 9 (2009)
Page No.: 31-37
Published: May 15, 2009
Section: Article
DOI: https://doi.org/10.32508/stdj.v12i9.2283
Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Download PDF = 616 times
Total = 616 times
Most read articles by the same author(s)
- Phuong Nhat Tran, Thuoc Linh Tran, Van Hung Pham, In vitro transfer of carbapenem resistant genes from Klebsiella pneumoniae to Escherichia coli J53 , Science and Technology Development Journal: Vol 19 No 1 (2016)
- Phuong Hoang Ngoc Nguyen, Phuoc Thanh Nguyen, Thang Luong Pham, Hoang Duc Nguyen, Thuoc Linh Tran, Trang Thi Phuong Pham, Using cellulose binding module of CBM3A as the purification tag for secreted Endoglucanase A (CelA) in Bacillus subtilis , Science and Technology Development Journal: Vol 19 No 2 (2016)
- Phuong Nhat Tran, Phuong Thi Kim Huynh, Trang Thi Phuong Tran, Thuoc Linh Tran, Van Hung Pham, Clonning and expression of recombinant carbapenemase KPC-2 enzyme in E. coli cytoplasm , Science and Technology Development Journal: Vol 19 No 2 (2016)
- Kim Thi Hong Tran, Thuoc Linh Tran, Synthesis and immunogenicity evaluating of a discontinuous B-cell epitope predicted from influenza virus A/H5N1 HA antigen , Science and Technology Development Journal: Vol 18 No 2 (2015)
- Nam Hoai Nguyen, Trang Thi Phuong Phan, Thuoc Linh Tran, Hoang Duc Nguyen, Cloning and investigating GFP (green fluorescent protein) allowing higher expression in Bacillus subtilis , Science and Technology Development Journal: Vol 18 No 2 (2015)
- Xuan Mai Huong Le, To Dinh Le, Thao Thi Phuong Dang, Thuoc Linh Tran, Cloning and expression of recombinant human leptin in Escherichia coli , Science and Technology Development Journal: Vol 16 No 1 (2013)
- Nam Hoai Nguyen, Trang Thi Phuong Phan, Thuoc Linh Tran, Hoang Duc Nguyen, Investigating the expression of GFP fused with HIS-TAG at N- or Cterminus using plasmid pHT253 and pHT254 in Bacillus subtilis , Science and Technology Development Journal: Vol 17 No 4 (2014)
- Thang Luong Pham, Trang Thi Phuong Phan, Thuoc Linh Tran, Hoang Duc Nguyen, Production of endoglucanase A of Clostridium thermocellum in Bacillus subtilis , Science and Technology Development Journal: Vol 17 No 4 (2014)
- Xuan Mai Huong Le, Khanh Cat Vuong, Hoa Thanh Tran, Giao Ngoc Quynh Nguyen, Thao Thi Phuong Dang, Thuoc Linh Tran, PRELIMINARY STUDY ON PRESERVATION OF RECOMBINANT hG-CSF BY LYOPHILIZATION , Science and Technology Development Journal: Vol 14 No 3 (2011)
- Duy Long Duong, Duc Van Luong, Hieu Thi Phuong Nguyen, Hoa Thanh Tran, Thao Thi Phuong Dang, Thuoc Linh Tran, EXPRESSION AND PURIFICATION OF RECOMBINANT T4 DNA LIGASE IN E. COLI , Science and Technology Development Journal: Vol 14 No 3 (2011)
