Downloads
Abstract
Traditional methods of recombinant protein purification are uneconomic and inconvenient to the secreted proteins at large-volume. CBM3a, a module from cellulosome’s scaffoldin of Clostridium thermocellum, directs the binding of the cellulase complex on the cheap cellulose substrate. Most of previous studies about CBM3a fused with cellulases as the purification tag were conducted in intracellular Escherichia coli system. In this research, we used the extracellular Bacillus subtilis WB800N expression system to investigate the CBM3a-tag fused with endoglucanase CelA into plasmid pHT. The results indicated that protein CelA was secrected and purified by CBM3a-tag binding on the Regenerated Amorphous Cellulose (RAC) subtrate. This can be used for further improvement in protein purification tag designing.
Issue: Vol 19 No 2 (2016)
Page No.: 5-14
Published: Jun 30, 2016
Section: Natural Sciences - Research article
DOI: https://doi.org/10.32508/stdj.v19i2.754
Download PDF = 988 times
Total = 988 times
Most read articles by the same author(s)
- Phuong Nhat Tran, Thuoc Linh Tran, Van Hung Pham, In vitro transfer of carbapenem resistant genes from Klebsiella pneumoniae to Escherichia coli J53 , Science and Technology Development Journal: Vol 19 No 1 (2016)
- Phuong Nhat Tran, Phuong Thi Kim Huynh, Trang Thi Phuong Tran, Thuoc Linh Tran, Van Hung Pham, Clonning and expression of recombinant carbapenemase KPC-2 enzyme in E. coli cytoplasm , Science and Technology Development Journal: Vol 19 No 2 (2016)
- Kim Thi Hong Tran, Thuoc Linh Tran, Synthesis and immunogenicity evaluating of a discontinuous B-cell epitope predicted from influenza virus A/H5N1 HA antigen , Science and Technology Development Journal: Vol 18 No 2 (2015)
- Nam Hoai Nguyen, Trang Thi Phuong Phan, Thuoc Linh Tran, Hoang Duc Nguyen, Cloning and investigating GFP (green fluorescent protein) allowing higher expression in Bacillus subtilis , Science and Technology Development Journal: Vol 18 No 2 (2015)
- Xuan Mai Huong Le, To Dinh Le, Thao Thi Phuong Dang, Thuoc Linh Tran, Cloning and expression of recombinant human leptin in Escherichia coli , Science and Technology Development Journal: Vol 16 No 1 (2013)
- Trang Thi Phuong Phan, Anh Le Tuan Nguyen, Hoang Duc Nguyen, Cloning and expression of LTB in Escherichia coli and Bacillus subtilis , Science and Technology Development Journal: Vol 16 No 1 (2013)
- Nam Hoai Nguyen, Trang Thi Phuong Phan, Thuoc Linh Tran, Hoang Duc Nguyen, Investigating the expression of GFP fused with HIS-TAG at N- or Cterminus using plasmid pHT253 and pHT254 in Bacillus subtilis , Science and Technology Development Journal: Vol 17 No 4 (2014)
- Thang Luong Pham, Trang Thi Phuong Phan, Thuoc Linh Tran, Hoang Duc Nguyen, Production of endoglucanase A of Clostridium thermocellum in Bacillus subtilis , Science and Technology Development Journal: Vol 17 No 4 (2014)
- Xuan Mai Huong Le, Khanh Cat Vuong, Hoa Thanh Tran, Giao Ngoc Quynh Nguyen, Thao Thi Phuong Dang, Thuoc Linh Tran, PRELIMINARY STUDY ON PRESERVATION OF RECOMBINANT hG-CSF BY LYOPHILIZATION , Science and Technology Development Journal: Vol 14 No 3 (2011)
- Duy Long Duong, Duc Van Luong, Hieu Thi Phuong Nguyen, Hoa Thanh Tran, Thao Thi Phuong Dang, Thuoc Linh Tran, EXPRESSION AND PURIFICATION OF RECOMBINANT T4 DNA LIGASE IN E. COLI , Science and Technology Development Journal: Vol 14 No 3 (2011)