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Abstract

Nocosomial pathogen Klebsiella pneumoniae is a gram - negative bacterium that carries multiple antimicrobial resistant genes. Conjugative method was used for investigating of gene transfer from clinical carbapenem-resistant K. pneumoniae isolates to a recipient E. coli J53 in vitro. Multiplex PCR & Real-time PCR was used for detection of transferable genes among these strains. Transconjugants showed resistance to multiple antibiotics due to the presence of ESBLs & AmpC -lactamase as well as carbapenemase encoding genes. This is the great concern in Vietnam because resistant E. coli may become part of the normal gut flora and thereby a notable source of infections among sick and healthy persons in healthcare settings and in the community.



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Issue: Vol 19 No 1 (2016)
Page No.: 36-44
Published: Mar 31, 2016
Section: Natural Sciences - Research article
DOI: https://doi.org/10.32508/stdj.v19i1.542

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Creative Commons License

Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 How to Cite
Tran, P., Tran, T., & Pham, V. (2016). In vitro transfer of carbapenem resistant genes from Klebsiella pneumoniae to Escherichia coli J53. Science and Technology Development Journal, 19(1), 36-44. https://doi.org/https://doi.org/10.32508/stdj.v19i1.542

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