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Abstract
Leptin, a peptide hormone, is produced by mature adipocytes and functions primarily in the hypothalamus to reduce food intake and body weight. Recombinant h-leptin has been shown to be effective in obesity treatment. To overexpression of recombinant human leptin in Escherichia coli, the human leptin gene (hob gene) was cloned into the vector pET-28a. When analysis expression of human leptin in E. coli BL21(DE3) strain, it was found that recombinant vector pET-hob expressed h-leptinproteins in cytoplasm, and mainly as insoluble inclusion bodies. This result will be the premise for researching to produce recombinant human leptin protein.
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Article Details
Issue: Vol 16 No 1 (2013)
Page No.: 5-12
Published: Mar 31, 2013
Section: Natural Sciences - Research article
DOI: https://doi.org/10.32508/stdj.v16i1.1391
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Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
How to Cite
Le, X., Le, T., Dang, T., & Tran, T. (2013). Cloning and expression of recombinant human leptin in Escherichia coli. Science and Technology Development Journal, 16(1), 5-12. https://doi.org/https://doi.org/10.32508/stdj.v16i1.1391
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