Open Access 
Downloads
Abstract
A recombinant Bacillus subtilis clone displaying hexahistidine oligopeptide (6x-His) was generated to enhance the adsorption of Ni2+ and Cu2+ ions for environmental treatment application with initial concentrations of 2ppm to 200ppm. The equilibrium amount of metal ions adsorbed onto the bacteria increased with increasing of pH from 4.0 to 6.0. The engineered clone was shown to have a Ni2+ and Cu2+ relative adsorption of 1.33 and 1.8 as compared to the control strain, as expected. Among the models tested, namely the Langmuir, Freundlich isotherms, the biosorption equilibrium for both Ni2+ and Cu2+ was best described by the Langmuir model. For the ligand binding with heavy metal ions, namely the one and two site saturation, one and two site saturation with nonspecific, one and two site competition on cell for both engineered and control strain was best revealed by one site saturation.
Issue: Vol 13 No 3 (2010)
Page No.: 19-28
Published: Sep 30, 2010
Section: Sciences of Earth and Environment - Research article
DOI: https://doi.org/10.32508/stdj.v13i3.2140
Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Download PDF = 322 times
Total = 322 times
Most read articles by the same author(s)
- Phuong Nhat Tran, Thuoc Linh Tran, Van Hung Pham, In vitro transfer of carbapenem resistant genes from Klebsiella pneumoniae to Escherichia coli J53 , Science and Technology Development Journal: Vol 19 No 1 (2016)
- Phuong Hoang Ngoc Nguyen, Phuoc Thanh Nguyen, Thang Luong Pham, Hoang Duc Nguyen, Thuoc Linh Tran, Trang Thi Phuong Pham, Using cellulose binding module of CBM3A as the purification tag for secreted Endoglucanase A (CelA) in Bacillus subtilis , Science and Technology Development Journal: Vol 19 No 2 (2016)
- Phuong Nhat Tran, Phuong Thi Kim Huynh, Trang Thi Phuong Tran, Thuoc Linh Tran, Van Hung Pham, Clonning and expression of recombinant carbapenemase KPC-2 enzyme in E. coli cytoplasm , Science and Technology Development Journal: Vol 19 No 2 (2016)
- Kim Thi Hong Tran, Thuoc Linh Tran, Synthesis and immunogenicity evaluating of a discontinuous B-cell epitope predicted from influenza virus A/H5N1 HA antigen , Science and Technology Development Journal: Vol 18 No 2 (2015)
- Nam Hoai Nguyen, Trang Thi Phuong Phan, Thuoc Linh Tran, Hoang Duc Nguyen, Cloning and investigating GFP (green fluorescent protein) allowing higher expression in Bacillus subtilis , Science and Technology Development Journal: Vol 18 No 2 (2015)
- Xuan Mai Huong Le, To Dinh Le, Thao Thi Phuong Dang, Thuoc Linh Tran, Cloning and expression of recombinant human leptin in Escherichia coli , Science and Technology Development Journal: Vol 16 No 1 (2013)
- Nam Hoai Nguyen, Trang Thi Phuong Phan, Thuoc Linh Tran, Hoang Duc Nguyen, Investigating the expression of GFP fused with HIS-TAG at N- or Cterminus using plasmid pHT253 and pHT254 in Bacillus subtilis , Science and Technology Development Journal: Vol 17 No 4 (2014)
- Thang Luong Pham, Trang Thi Phuong Phan, Thuoc Linh Tran, Hoang Duc Nguyen, Production of endoglucanase A of Clostridium thermocellum in Bacillus subtilis , Science and Technology Development Journal: Vol 17 No 4 (2014)
- Xuan Mai Huong Le, Khanh Cat Vuong, Hoa Thanh Tran, Giao Ngoc Quynh Nguyen, Thao Thi Phuong Dang, Thuoc Linh Tran, PRELIMINARY STUDY ON PRESERVATION OF RECOMBINANT hG-CSF BY LYOPHILIZATION , Science and Technology Development Journal: Vol 14 No 3 (2011)
- Duy Long Duong, Duc Van Luong, Hieu Thi Phuong Nguyen, Hoa Thanh Tran, Thao Thi Phuong Dang, Thuoc Linh Tran, EXPRESSION AND PURIFICATION OF RECOMBINANT T4 DNA LIGASE IN E. COLI , Science and Technology Development Journal: Vol 14 No 3 (2011)
