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TESTING PROTOCOLS FOR TRANSFORMATION OF PHALAENOPSIS AMABILIS L. BY BIOLISTIC AND AGROBACTERIUM TUMEFACIENS

Tran Le Luu Ly 1
Nguyen Huu Hoang 1
Bui Lan Anh 1
Tran Nguyen Vu 1
Bui Van Le 1
Volume & Issue: Vol. 11 No. 10 (2008) | Page No.: 109-113 | DOI: 10.32508/stdj.v11i10.2708
Published: 2008-10-31

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Copyright The Author(s) 2023. This article is published with open access by Vietnam National University, Ho Chi Minh city, Vietnam. This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0) which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. 

Abstract

We have succeeded in testing protocols for producing transgenic plant using Agrobacterium tumefaciens and microprojectile bombardment for PLB laminae of Phalaenopsis amabilis L. cultured in stationary liquid medium. In mediated transformation, A.tumefaciens C58pGV2260 containing p35SGUS-INT with the uidA gene and nptII gene was used, and maximum level of gus expression was observed 4 days ater co-cultivation with acetosyringone concentration was 50µM. In direct gene transformation, we used BiolisticTM PDS-1000/He system to transfer pBAR-GUS (containing uidA gene and nptII gene) into plant cells. The result showed that PBL had the highest GUS activity at 6 cm fire distance with 500 μg tungsten and 0,5 μg DNA mix.

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