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Abstract

We have succeeded in testing protocols for producing transgenic plant using Agrobacterium tumefaciens and microprojectile bombardment for PLB laminae of Phalaenopsis amabilis L. cultured in stationary liquid medium. In mediated transformation, A.tumefaciens C58pGV2260 containing p35SGUS-INT with the uidA gene and nptII gene was used, and maximum level of gus expression was observed 4 days ater co-cultivation with acetosyringone concentration was 50µM. In direct gene transformation, we used BiolisticTM PDS-1000/He system to transfer pBAR-GUS (containing uidA gene and nptII gene) into plant cells. The result showed that PBL had the highest GUS activity at 6 cm fire distance with 500 μg tungsten and 0,5 μg DNA mix.



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Issue: Vol 11 No 10 (2008)
Page No.: 109-113
Published: Oct 31, 2008
Section: Natural Sciences - Research article
DOI: https://doi.org/10.32508/stdj.v11i10.2708

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Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 How to Cite
Luu Ly, T., Huu Hoang, N., Lan Anh, B., Nguyen Vu, T., & Van Le, B. (2008). TESTING PROTOCOLS FOR TRANSFORMATION OF PHALAENOPSIS AMABILIS L. BY BIOLISTIC AND AGROBACTERIUM TUMEFACIENS. Science and Technology Development Journal, 11(10), 109-113. https://doi.org/https://doi.org/10.32508/stdj.v11i10.2708

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