ISOLATING A BIOACTIVE QUINONE FROM IN VITRO CULTURED DROSERA BURMANNI VAHL

Abstract

To establish in vitro cultivation of Drosera burmanni Vahl, steriled seeds were germinated on MS basal medium but decreasing of 1/2 macro salts content (MS 1/2) supplemented with saccharose (30 g/l), activated carbon (1 g/l), Casein hydrosylate (100 mg/l) at pH 5.8. Stem node of D. burmanni Vahl were cultured on MS 1/2 medium (solid and liquid) with saccharose (30 g/l), activated carbon (1 g/l), Casein hydrosylate (100 mg/l) at pH 5.8. A quinone compound was isolated from ethanol extract of dry plants. Bioassays were used to detect the activities of this compound. A quinone compound was identified by mass spectrum, IR spectrum and 1H-, 13 C-NMR spectrum.