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Abstract
Green callus clusters inducted from in vitro Catharanthus roseus leaf explant have been cultured in a modified Murashige and Skoog (MS) liquid induction medium supplemented with different plant growth regulators containing auxin and cytokinine. The indution medium with 1 mgloc-naphthaleneacetic acid (NAA) and 0.5 mgl' kinetin (Kin) gave the greatest biomass and total alkaloid, meanwhile in the same medium containing 2,4 dichlorophenoxyacetic acid (2,4-D) instead of NAA reduced biomass and total alkaloid production. The optimum saccharose concentration for alkaloid and biomass was 60 gl. A combination between optimum plant growth and saccharose concentration in C. roseus suspension culture gave more vincristine alkaloid than natural C. roseus leaf.
Issue: Vol 9 No 6 (2006)
Page No.: 59-66
Published: Jun 30, 2006
Section: Article
DOI: https://doi.org/10.32508/stdj.v9i6.2921
Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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