THE OBTAINMENT OF THE FUNGICIDES BELONGING TO NAPHTOQUINONE GROUP FROM IMPATIENS BALSAMINA L. USING CELL AND TISSUE CULTURES

Abstract

Attempts were made in this paper to show the presence of the fungicides belonging to naphtoquinone group in some cultivars of Impatiens balsamina L. Calluses were initiated from seeds and cotyledons on B5 medium containing 2 mg/l NAA and 1 mg/l kinetin. Cell suspensions were obtained from these calluses, using B5 medium supplemented with 0,1 mg/l 2,4-D and 1 mg/l kinetin. The fungicides and the plant growth regulators were extracted from the leaves and the calluses. Bioassays were used to detect the presence of hormone and fungicide activities. Roles of the plant growth regulators on the callus growth in relation to fungicide formation were discussed.