IN VITRO PLANT REGENERATION FROM BULB SCALE SECTIONS OF LILIUM LONGIFLORUM THUNB.

Abstract

Plant regeneration from bulb scale sections of Lilium longiflorum Thunb. was investigated. The explants were cultured on MS medium supplemented with the various combinations of auxin and cytokinin (NAA and BA). The interaction of NAA and BA on new bulblet and root formation was examined. The results of the investigation showed that maximum shoot development could be achieved on MS basal medium supplemented with 1 mg/l BA in combination with 1 mg/l NAA using bulb scale sections as explant. The bud primordia were observed at the surface of scale cuttings, after 12 days in culture. The explants underwent intensive direct organogenesis which could lead to the formation of shoots. An increase in auxin and cytokinin content and respiratory intensity, and a decrease in gibberellin and abscisic acid content were observed during this shoot formation. The bulblets obtained were transferred to MS medium supplemented with 2 mg/l IAA to facilitate the regeneration of normal roots.