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Abstract
Tranformation of fish by-products to food and products with bioactivity brings economic benefits and reduces waste ratio in fishsery industry. This study focussed on the extraction and purification of protease from intestine of Basa catfish (Pangasius bocourti). The optimal conditions for protease extraction from Basa catfish intestine were as follows: intestine and buffer ratio-1/1(w/w); pH 9,5; temperature 35oC; extraction time is 10 minutes. Isopropanol was a suitable precipitating agent for protease purification. The optimal ratio of enzyme extract and iosopropanol was 15/85 (v/v). The alkaline protease recovery yield was 90,42% and the purity degree was 1,65.
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Article Details
Issue: Vol 11 No 11 (2008)
Page No.: 57-64
Published: Nov 30, 2008
Section: Sciences of Earth and Environment - Research article
DOI: https://doi.org/10.32508/stdj.v11i11.2730
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Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
How to Cite
Hien, T., & Viet Man, L. (2008). EXTRACTION AND PURIFICATION OF PROTEASE FROM BASA CATFISH INTESTINE (Pangasius bocourti). Science and Technology Development Journal, 11(11), 57-64. https://doi.org/https://doi.org/10.32508/stdj.v11i11.2730
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