Open Access 
Downloads
Abstract
This research focussed on brewer yeast autolysis for invertase extraction. Firstly, classical method was used for determination of autolytic conditions of One parameter was varied while the others were fixed. The results were as follows: ratio of yeast and solven (acetate buffer): 1/6 (w/w), autolytic temperature: 50°C, initial pH: 5.5 and autolytic time: 50 hours. In these conditions, the recovery invertase activity was 94.7 activity units/gram of yeast dry matter. Then, the central composite design method was used to optimize the temperature and initial pH factors of brewer yeast autolysis for invertase extraction. Our results showed that optimal temperature and pH for yeast autolysis were 45°C and 5.5 respectively. The obtained invertase activity in the autolysate was 120.9 units/gram of yeast dry matter; the specific activity of the crude invertase was 0.7 units/mg protein.
Issue: Vol 9 No 12 (2006)
Page No.: 49-56
Published: Dec 15, 2006
Section: Article
DOI: https://doi.org/10.32508/stdj.v9i12.3131
Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Download PDF = 398 times
Total = 398 times
Most read articles by the same author(s)
- Le Van Viet Man, Tran Quoc Huy, STUDY ON PROLONGATION OF WATERMELON FRESH-CUT SHELFLIFE BY OZONE TREATMENT , Science and Technology Development Journal: Vol 11 No 9 (2008)
- Ton Nu Minh Nguyet, Le Ngoc Lieu, Nguyen Thi Hien Luong, Le Van Viet Man, EFFECT OF INITIAL SULFUR DIOXIDE CONTENT IN MUST ON THE KINETICS OF WINE PRIMARY FERMENTATION, USING YEAST IMMOBILIZED IN CALCIUM ALGINATE GEL , Science and Technology Development Journal: Vol 11 No 9 (2008)
- Bui Thanh Huyen, Le Van Viet Man, ALCOHOLIC FERMENTATION WITH DIFFERENT INITIAL GLUCOSECONCENTRATION USING IMMOBILIZED YEAST IN CALCIUM ALGINATE GEL , Science and Technology Development Journal: Vol 11 No 12 (2008)
- Ton Nu Minh Nguyet, Nguyen Thi Hien Luong, Le Ngoc Lieu, Le Van Viet Man, INFLUENCE OF INITIAL TANNIN CONCENTRATION IN MUST ON THE KINETICS OF WINE FERMENTATION, USING YEAST IMMOBILIZED IN CALCIUM ALGINATE GEL , Science and Technology Development Journal: Vol 11 No 12 (2008)
- Tran Quoc Hien, Le Van Viet Man, EXTRACTION AND PURIFICATION OF PROTEASE FROM BASA CATFISH INTESTINE (Pangasius bocourti) , Science and Technology Development Journal: Vol 11 No 11 (2008)
- Lai Quoc Dat, Le Van Viet Man, Nguyen Nhu Y, STUDY ON PROBABILITY OF SYRUP PRE-CONCENTRATION BY NANOFILTRATION , Science and Technology Development Journal: Vol 10 No 4 (2007)
- Le Van Viet Man, Pham Quoc Chuong, IMPROVEMENT OF FERMENTATION PERFORMANCE IN HIGH GRAVITY BREWING , Science and Technology Development Journal: Vol 10 No 6 (2007)
- Vu Thi Kim Hanh, Le Van Viet Man, STUDY ON CONTINUOUS HYDROLYSIS OF SUCROSE BY INVERTASE IMMOBILIZED IN ALGINATE GEL , Science and Technology Development Journal: Vol 10 No 11 (2007)
- Lai Quoc Dat, Le Van Viet Man, Vo Thi Luyen, STUDY ON INFLUENCE OF SOME TECHNOLOGICAL FACTORS ON HIGH GRAVITY BREWING , Science and Technology Development Journal: Vol 9 No 4 (2006)
- Tran Quoc Hien, Le Van Viet Man, EXTRACTION AND PURIFICATION OF PROTEASE FROM BASA CATFISH INTESTINE (Pangasius bocourti) , Science and Technology Development Journal: Vol 9 No 11 (2006)
